This is done under a general anaesthetic. The doctors pass a needle through the skin between the scrotum (the skin that covers the testicles) and the anus. They use a needle which can take many small tissue samples from different areas of the prostate. This test is used if your doctor thinks there is a high risk you have cancer, but biopsies have not found it.
Your doctor can talk to you about whether a template biopsy might be helpful in your situation.